Both structurally related and unrelated compounds may cause false-positive assay results by binding
nonspecifically to the antibodies used in a particular immunoassay.
These low-abundant species may be bound specifically or
nonspecifically to the depletion ligand, depletion target protein (e.g., carrier proteins), or the solid support(s).
To rule out the possibility that the TaqMan MicroRNA Assay
nonspecifically detects any contaminating genomic DNA in RNA preparations, we treated an RNA preparation with different combinations of DNase I and/or RNase A and assayed for miR-141, which was known to exist at a detectable concentration in this RNA preparation before any treatment.
We washed the beads 6 times with [PBSO.sub.0.25]-Tx to remove unbound and
nonspecifically bound proteins.
The differentiation between specifically and
nonspecifically bound mass is accomplished by differencing side-by-side target and reference protein spots.
The present study used a capture approach involving biotinylated antibodies immobilized to streptavidin-conjugated beads, but the method is not limited to this approach; we have successfully quantified antigens with capture strategies involving antibodies covalently attached or
nonspecifically adsorbed to beads and membranes (data not shown).
The immunosuppressive drugs are effective at preventing rejection over the short term; however, because anti-rejection drugs suppress the immune system
nonspecifically, people taking these drugs face the risk of serious infections and even cancer.
[17] Pregnant women are at increased risk of developing TB disease, [19] but may present
nonspecifically and/or have overlap of TB symptoms (fatigue and malaise) with normal pregnancy-related complaints, making the clinical diagnosis of TB in pregnancy difficult.
The menu is short and
nonspecifically Asian, with guest appearances from halloumi, haggis and maple syrup.
For example, "4" and "2" copies were detected by the nonspecific, STRC.ex02 and STRC.ex10, assays in the STRC normal and homozygous deletion samples, respectively, demonstrating that these assays were
nonspecifically quantifying the 2 copies from each STRC and pSTRC (i.e., 4 total copies).
These metastases often present as
nonspecifically, as abdominal pain, nausea, vomiting, diarrhea, and changes in bowel habits [5, 6].
She comes around the counter with a hopeful, if nervous, smile and gestures
nonspecifically. "Sergeant, can I get you some water while you wait?