Para ello se evaluo el patron de union y distribucion de los espermatozoides a la ZP mediante Microscopia Electronica de Barrido (MEB) y el estado
acrosomal mediante microscopia de fluorescencia.
Non- significant results of percentage spermatozoa motility viability
acrosomal integrity PMI and lipid peroxidation may be correlated to the fact that L- cysteine is a water soluble antioxidant and due to this characteristic do not bind properly to plasma membrane (Khalifa et al.
Hyaluronic acid binding by human sperm indicates cellular maturity, viability, and un-reacted
acrosomal status.
This result showed that L-carnitine supplementation in freezing extender could not protect cat cryopreserved epididymal spermatozoa against
acrosomal damage.
WGA detects GlcNac residues on the glycocalyx located on the
acrosomal membrane surface.
The great amounts of polyunsaturated fatty acids found in H2 samples could be related to the loss of
acrosomal integrity.
It was demonstrated in the abalone that the sperm
acrosomal lysin binds to the vitelline envelop receptor and creates a hole through which sperm could pass and fuse with the egg cell membrane, and this occurs by a nonenzymatic species-specific mechanism (Lewis et al.
ZP3 is believed to mediate the initial binding of sperm and the acrosome reaction, while ZP2 is involved in adhesion to the sperm's inner
acrosomal membrane (Dell et al., 1999).
Ademas la tincion con eosina/negrosina, preferiblemente en un microscopio de contraste de fase, permite evaluar el estado
acrosomal de espermatozoides vivos y muertos.
Validation of an
acrosomal stain for equine sperm that differentiates between living and dead sperm.