separating funnel

n-Hexane extracts: The dried extract of each sample was taken in a beaker and 100 mL of methanol was added in each, shaken well and transferred to 250 ml separating funnel. 100 mL of n-hexane was added to the separating funnel and mixture was left for 10 minutes stand-by.

The extract solution was inserted into a separating funnel, and then, the n-hexane solvent was added with a volume ratio of 1:1 with the aquades.

30 mL of tea-cooled beverage was put into a separating funnel of 60 mL, and then 30 mL of ethyl acetate was added.

(12) A total of 5 g methanol extract was dissolved with methanol (Merck, Darmstadt, Germany) and filtered into a separating funnel. Diethyl ether (Merck, Darmstadt, Germany) was then added until the formation of white precipitation.

The filtrated mixture was then mixed with 20 mL 0.05% sulfuric acid in a separating funnel for 20 minutes and the lower layer was collected.

A 5L separating funnel was used for fractionation of the crude methanolic extract of the plant (1.32 kg).

The mixtures were transferred into a separating funnel. From the separating funnel, the lower layer that formed the organic layer was collected and evaporated to dry.

Among all germinated legumes, cowpea demonstrated the most elevated amount of total antioxidant activity (98.1%) when either filter paper or separating funnel was utilized.

Separating funnel, Centrifuge, homogenizer, Scales, funnel and filter paper, Distillation, Evaporators, gas chromatography unit, mixer, gas stove.

A mixture (1:1) of ethylacetate and n-hexane was placed in separating funnel containing 10 mL aqueous solution of parathion.

The mixture was immediately transferred into a separating funnel and closed tightly.