For SDS-PAGE and Western blot, whole bacterial cell extracts or flagella extracts were denatured and separated in 14%
polyacrylamide gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore Corp., Bedford, MA) (27).
Isoelectric focusing was on linear, wide-range immobilized
polyacrylamide gel strips (17 cm; pH 3-10; Bio-Rad Laboratories) in the first dimension and on 15%
polyacrylamide gels (20 cm x 22 cm x 1 mm) in the second dimension.
Eight percent
polyacrylamide gels (Invitrogen) were also suitable in cases where primers C and D were used for the determination of the Hp 2 allele instead of primers A and B.
The digests were fractionated on nondenaturing 10%
polyacrylamide gel. The Bst EII pattern generated during PRA yielded two 235/210-bp bands similar to the patterns attributed to M.
DGGE was carried out essentially as described previously (12) with 20 [micro]L of sample loaded onto a 10%
polyacrylamide gel containing a 40-70% denaturing gradient (exon 2b); 8%
polyacrylamide gel containing a 25-65% or a 40-70% denaturing gradient (exons 3, 4, 5, and 6); 12% polyacrylamide gradient gel containing a 40-80% denaturing gradient (exon 2a, 3'-UTR).
Catalysts' for
polyacrylamide gel polymerization and detection of proteins by silver staining.
DNA bands on the
polyacrylamide gel were visualized by silver staining (Bio-Rad).
The different conformers are separated on a nondenaturing
polyacrylamide gel and detected either radioactively or with silver staining.
Fact.MR has announced the addition of the "
Polyacrylamide Gel Electrophoresis (PAGE) Market Forecast, Trend Analysis & Competition Tracking - Global Market Insights 2018 to 2026"report to their offering.
A sodium dodecyl sulphate
polyacrylamide gel electrophoresis profile demonstrated that the [alpha]' and [alpha] subunits of [beta]-conglycinin and the acidic chains of glycinin gradually degraded.
However the method is performed and compared for quality mostly in
polyacrylamide gel to detect denatured Single stranded DNA9,10.