acetonitrile

For this purpose 200 ug TSM (10 x recommended application rate) dissolved in 1 mL of water and acetonitrile (50:50; v/v) was applied to the soil column.

Supernatant layer (organic layer) was collected in a separate culture tube, was dried under nitrogen at 60AdegC and then reconstituted with 200 ul deionized water (DH2O) and acetonitrile, in equal quantity.

USP guidelines were used to determine the suitability of system which consists of optimized conditions, i.e., merging of ammonium acetate buffer solution pH 7.6 and acetonitrile 50:50% v/v as mobile phase at a flow rate of 0.5 mL/min over Agela C18, 125 mm x 4.6 mm, 5 um.

Raw chitosan was allowed to swell in the acetonitrile solvent at room temperature, and then (CH3)3CO-K+ dissolved in acetonitrile was added, and stirred for 24 h.

The mobile phase was composed of acetonitrile and 25 mM phosphate buffer (pH 3.00) (75:25, v/v).

The electronic absorption spectra of pyrazoloisoindol derivative ligand and its [Ag.sup.+], [Co.sup.2+], [Hg.sup.2+], [Zn.sup.2+], [Ni.sup.2+], [Cu.sup.2+], [Cd.sup.2+], [Mn.sup.2+], [Cr.sup.3+], [Fe.sup.3+] and [Pb.sup.2+] complexes in acetonitrile are shown in Figure 2.

Average size of nanoparticles was calculated about 9.42nm in 2-propanol, 6.36nm in acetonitrile and 16.05nm in n-Hexane which are close to the DLS Analysis.

All HPLC-grade solvents (acetonitrile, methanol, glacial acetic acid, and triethylamine) were obtained from Fisher Scientific (Pittsburgh, PA, USA) and Merck Taiwan Ltd.

Thermal melting ([T.sub.M]) experiments were done at pH 5.4 on modified oligonucleotides in the absence or presence of KCl alone or with the dehydrating agent, acetonitrile (ACN) (Table 3).

The electronic absorption spectra of ring-opened isomers 1-4 O in acetonitrile are characterized by long-wavelength bands with maxima in the range of 321-364 and 351-380 nm as shown in the example of 4 (Figure 1, Table 1).

Silicon wafers (SUMCO Corporation, Japan), methanol (99.7%, Sigma-Aldrich, USA), ethanol (95%, Sigma-Aldrich, USA), acetonitrile (99.8%, Sigma-Aldrich, USA), chloroform (E-Merck, Germany), carbon tetrachloride (Qualikems, India), isopropanol (Panreac Quimica Co., Spain), benzene (E-Merck, Germany), dioxane (Fisher Scientific Co., UK), hexane (99.9%, Avonchem, UK), hydrofluoric acid (THF, Panreac Quimica Co., Spain), tetrahydrofuran (THF) (Nexgen Chemicals, India), hydrogen peroxide ([H.sub.2][O.sub.2]) (Avonchem, UK), dimethyl sulphoxide (DMSO) (99% Fluka Chemika, USA), dimethylformamide (DMF) (99.8%, Panreac Quimica Co., Spain), chloroplatinic solution, and distilled water (DI) were of analytical grade and used directly as received without any further purification.