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Embryonated chicken eggs were used by Koprowski and Cox for serial passage of the Flury strain. The virus was initially passaged 136 times in 1 day old chicks followed by passage in embryonated chicken egg.
They were represented by Flury strains low egg passage and high egg passage, originally isolated from a person in Georgia in 1939 (25) and by Street-Alabama- Dufferin B19 and Evelyn Rokitniki Abelseth derivatives, obtained from a rabid dog in Alabama in 1935 (26).
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