The use of LAB for protective culture has some advantages on
bacteriocin and its other antimicrobial metabolites, as the living LAB can adopt itself to the changing environment and conditions of foods during processing and storage, producing antimicrobials and other metabolites constantly (Settanni and Corsetti, 2008).
Possibility of usage of lactic acid bacteria (LAB) as substrates of other chemical preservatives of food and production of
bacteriocin which is of great economic interest had been reported earlier (Admas, 1999).
Bacteriocin antiseptic drug has been produced in this research by using lactobacillus, and it has been stabilized on the produced nanofibers at the same time with the beginning of electrospinning process.
Amino acid and nucleotide sequence, adjacent genes, and heterologous expression of hiracin JM79, a sec-dependent
bacteriocin produced by Enterococcus hirae DCH5, isolated from Mallard ducks (Anasplatyrhynchos).
Antimicrobial activity and occurrence of
bacteriocin structural genes in Enterococcus spp.
Diverse mechanisms describing the
bacteriocin mode of action were outlined but the cell membrane still remains the main target of these AMPs.
Initially the isolate was screened for its ability to produce biosurfactant and
bacteriocin using various qualitative and quantitative methods.
Plates were incubated for additional 18h and then checked for clear inhibition zones around spots of the presumed
bacteriocin producers.
Next, the culture was centrifuged at 5500 rpm (9000g) for 5 -10 min and the clear supernatant was used as an unpurified
bacteriocin extract.
If inhibition zone were found in the third well, the isolates were considered to be able to produce
bacteriocin or
bacteriocin like substances) (18).