celloidin

These barriers include celloidin tubes [1], interposition of tunica vaginalis [2], cellophane [35], vitallium [6], fatty tisues, muscle and fascia [7], endothelial cuffs [8], veingrafts [9], stainless steel implants [10], monomolecular cellulose filter tubes [11], gastroecnemius muscle flap [12], stainless steel tubes [11,13], silicone-dacrone reinforced [14], polytetrafluroethylene (PTFE) [15], polyethylene tubes [16], silicone sheath [17], bovine fascia [18] and dacron tube [18].

For histological studies, a biopsy at injured site was conducted and fixed them in 10% solution of neutral formalin, decalcified in 4% solution of nitric acid, dehydrated in alcohols of increasing strength and embedded in celloidin, according to the technique of Sarkisov et al.

Tissues were dehydrated and embedded in paraffin using the Double Embedding Method: 80 per cent ethanol--90 per cent Ethanol--96 per cent ethanol--99 per cent ethanol--99 per cent ethanol/Methyl Salicylate--Methyl Salicylate--1 per cent Celloidin in Methyl Salicylate--Methyl Salicylate/Paraffin--several changes of paraffin; and finally embedded in paraffin.