Interestingly, CENP-E is involved in the initial alignment of chromosomes at the spindle equator on the metaphase plate, and therefore, it is required for steady spindle microtubule capture at the kinetochores, which is a critical step in proper chromosome
congression during prometaphase.
Microinjection of serum containing ACA into mouse oocytes could hinder chromosome
congression and cause meiotic arrest in interphase or mitotic arrest in prometaphase [8].
Kinetochore motors drive
congression of peripheral polar chromosomes by overcoming random arm-ejection forces.
Eric Olivarez has sought a
congression commendation for an honest security guard who earlier this year returned an envelope containing over P1 million to its rightful owner.
At a
congression of some of the world's top chefs every year in Spain
Among them, PLK1 is best studied which is essential mitotic kinase that controls mitotic entry, centrosome maturation, bipolar spindle formation, cohesion dissociation, and chromosome
congression and segregation, as well as cytokinesis [4-6].
Over the years, many methods have been developed for preparing somatic chromosomes, and they are generally made up of four stages: first, the seed is germinated and the actively growing root tip is collected; second, a pretreatment using ice cold water for a definite period of time or 8-Hydroxyquinoline, Colchicine, Paradichlorobenzene, or [alpha]-monobromonaphthalene for a definite period of time prior to fixation to inhibit spindle formation, preventing the
congression of chromosomes to the metaphase plate; third is fixation in any of a large range of solutions; fourth, preparation of the root tips by hydrolysis, followed by staining.
However, it was the study of the large living cells of Orthoptera and their micromanipulation, that helped resolve this problem and furthermore, disentangle the complex processes of chromosome attachment to the spindle, and their
congression, orientation and segregation in mitosis and meiosis.
(2003) reported that BPA from cracked polycarbonate caging caused meiotic aneuploidy in genetically susceptible female mice, and dosing this strain with 20 [micro]g/kg/day BPA for 7 days caused chromosome
congression failure (disturbed metaphase chromosome alignment) during oocyte meiosis.